AXIN1 Antibody / Wnt Signaling Scaffold Protein Antibody (FY13148)

AXIN1 Antibody HeLa Cell IF. Immunofluorescent staining of HeLa cells using AXIN1 Antibody / Wnt Signaling Scaffold Protein Antibody demonstrates predominantly cytoplasmic green fluorescence throughout the cellular compartment, consistent with the established localization of Axis Inhibition Protein 1 (AXIN1) as a scaffold protein within the Wnt/beta-catenin signaling pathway. Co-staining with Beta Tubulin (red) highlights the cytoskeletal network and provides cellular structural reference. The observed AXIN1 staining pattern is consistent with its role in assembling the beta-catenin destruction complex and coordinating intracellular signal transduction events that regulate cellular differentiation, proliferation, and developmental signaling. Enzyme antigen retrieval was performed for 15 minutes prior to staining. Cells were incubated with primary antibodies overnight at 4oC followed by fluorescent secondary antibody detection and visualization by fluorescence microscopy.

AXIN1 Antibody Multi-Species WB. Western blot analysis of human HT-1080, human A549, human K562, mouse brain, and mouse thymus lysates using AXIN1 Antibody / Wnt Signaling Scaffold Protein Antibody demonstrates a predominant immunoreactive band migrating slightly above 100 kDa across all samples tested. The observed molecular weight is consistent with the expected size of Axis Inhibition Protein 1 (AXIN1), a scaffold protein that serves as a central regulator of the Wnt/beta-catenin signaling pathway. AXIN1 frequently migrates at a higher apparent molecular weight than predicted due to extensive phosphorylation, a known regulatory mechanism that influences protein stability and signaling activity. Additional weaker bands observed above and below the major species may represent post-translationally modified forms, ubiquitinated species, or limited proteolytic products. Detection of AXIN1 in both human and mouse samples is consistent with the highly conserved role of this protein in developmental signaling, beta-catenin regulation, cellular differentiation, and tissue homeostasis.