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Home >> Antibodies >> Aurora B Antibody

Aurora B Antibody (R30904)

  Catalog No Formulation Size Price (USD)  
Image R30904 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 389
Western blot testing of Aurora B antibody and Lane 1: rat liver; and human samples 2: 22RV; 3: HeLa; 4: SW620 cell lysate. Predicted molecular weight: 39-45 kDa
Availability 1-3 business days
Species Reactivity Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt O70126
Applications Western blot : 0.5-1ug/ml
Limitations This Aurora B antibody is available for research use only.
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Aurora kinase B, also called Aik2, AIM-1, ARK2, AurB, STK12, IPL1, PPP1R48 or STK5, localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. Cell cycle and Northern blot analyses showed that AURKB is expressed in the S phase and persistently thereafter. Western blot analysis indicated that it is localized in the midbodies during anaphase. Northern blot analysis detected strong expression of a 1.5-kb transcript in thymus, with weaker expression in small intestine, testis, colon, spleen, and brain. Examination of the role of both kinases in the phosphorylation of CENPA revealed that the reaction is mediated sequentially by AURKA and AURKB in early mitosis. EB1 overexpression enhanced AURKB kinase activity, and knockdown of EB1 with small interfering RNA impaired AURKB activity. EB1 protected Aurora B from dephosphorylation/inactivation by protein phosphatase-2A by blocking binding of PP2A.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the Aurora B antibody may be required due to differences in protocols and secondary/substrate sensitivity.


An amino acid sequence from the N-terminus of mouse AURKB (QNKQPFTIDNFEIGRP) was used as the immunogen for this Aurora B antibody.


After reconstitution, the Aurora B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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