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Home >> Antibodies >> Aurora B Antibody

Aurora B Antibody (F50225)

  Catalog No Formulation Size Price (USD)  
Image F50225-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 329
Image
F50225-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 135
Western blot analysis of Aurora B antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AURKB gene (2).
Aurora B antibody used in western blot to detect STK12/AuroraB in mouse spleen tissue lysate. Predicted molecular weight: 39-45 kDa
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt Q96GD4
Applications Western blot : 1:1000
IHC (Paraffin) : 1:50-1:100
Limitations This Aurora B antibody is available for research use only.
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Description

Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. STK12 (Aurora kinase B) localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A localizes to centrosomes

Application Notes

Titration of the Aurora B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 283-313 from the human protein was used as the immunogen for this Aurora B antibody.

Storage

Aliquot the Aurora B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Alternate Names

ARK, STK12

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