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Home >> Antibodies >> Aurora-A Antibody

Aurora-A Antibody (F50015)

  Catalog No Formulation Size Price (USD)  
Image F50015-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 329
Image
F50015-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 135
Western blot analysis of Aurora-A antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected with the Aurora-A gene (2). Predicted molecular weight ~45 kDa.
Confocal immunofluorescent analysis of Aurora-A antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
Aurora-A antibody flow cytometric analysis of HeLa cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt O14965
Applications Western blot : 1:1000
Immunofluorescence : 1:10-1:50
Flow Cytometry : 1:10-1:50
Limitations This Aurora-A antibody is available for research use only.
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Description

Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases, members of the Ser/Thr protein kinase family, associate with microtubules during chromosome movement and segregation. Auroria kinase A may play a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. It may be involved in microtubule formation and/or stabilization. This protein has also been postulated to play a key role during tumor development and progression. Aurora kinase A localizes on centrosomes in interphase cells and at each spindle pole in mitosis. It is highly expressed in testis, weakly in skeletal muscle, thymus and spleen, and also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines. Expression is cell-cycle regulated, low in G1/S, accumulates during G2/M, and decreases rapidly afterward. Defects in Aurora kinase A are responsible for numerical centrosome aberrations including aneuploidy.

Application Notes

Titration of the Aurora-A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 364-392 from the human protein was used as the immunogen for this Aurora-A antibody.

Storage

Aliquot the Aurora-A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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