AUF1 Antibody | RQ5629 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	RQ5629	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	429
Formulation

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Immunofluorescent staining of FFPE human intestinal cancer with AUF1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Immunofluorescent staining of FFPE human A431 cells with AUF1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

IHC staining of FFPE human lung cancer with AUF1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human renal cancer with AUF1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human B-lymphocytoma with AUF1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE mouse intestine with AUF1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat intestine with AUF1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of human 1) placenta, 2) HEK293, 3) HL-60, 4) K562, 5) A431, 6) HepG2 and 7) Caco-2 lysate with AUF1 antibody. Expected molecular weight: multiple bands from 37-45 kDa.

Western blot testing of 1) rat brain, 2) rat thymus, 3) mouse brain, 4) mouse thymus and 5) mouse SP2/0 lysate with AUF1 antibody. Expected molecular weight: multiple bands from 37-45 kDa.

Availability	1-3 business days
Species Reactivity	Human, Mouse, Rat
Format	Antigen affinity purified
Clonality	Polyclonal
Isotype	Rabbit IgG
Purity	Affinity purified
Buffer	Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt	Q14103
Localization	Nuclear
Applications	Western blot : 0.5-1ug/ml Immunohistochemistry : 1-2ug/ml Immunofluorescence : 2-4ug/ml Flow cytometry : 1-3ug/million cells Direct ELISA : 0.1-0.5ug/ml
Limitations	This AUF1 antibody is available for research use only.
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Description

Heterogeneous nuclear ribonucleoprotein D0 (HNRNPD) also known as AU-rich element RNA-binding protein 1 (AUF1) is a protein that in humans is encoded by the HNRNPD gene. It is mapped to 4q21.22. This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants.

Application Notes

Optimal dilution of the AUF1 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids E88-N246) was used as the immunogen for the AUF1 antibody.

Storage

After reconstitution, the AUF1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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AUF1 Antibody / hnRNP D / HNRNPD (RQ5629)

Description

Application Notes

Immunogen

Storage

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Area of Research

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Protocols

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