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Home >> Antibodies >> ATP5O Antibody

ATP5O Antibody (F54457)

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  Catalog No Formulation Size Price (USD)  
Image F54457-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54457-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC testing of FFPE human skeletal muscle tissue with ATP5O antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC testing of FFPE human skeletal muscle tissue with ATP5O antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of FFPE human skeletal muscle with ATP5O antibody.
Western blot testing of human HEK293 cell lysate with ATP5O antibody. Predicted molecular weight ~23 kDa.
Flow cytometry testing of human HEK293 cells with ATP5O antibody; Blue=isotype control, Green= ATP5O antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt P48047
Localization Cytoplasmic
Applications Western blot : 1:500-1:2000
Flow cytometry : 1:25 (1x10e6 cells)
Immunofluorescence : 1:25
Immunohistochemistry (FFPE) : 1:25
Limitations This ATP5O antibody is available for research use only.
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Description

ATP5O is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. This protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance.

Application Notes

The stated application concentrations are suggested starting points. Titration of the ATP5O antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 38-64 from the human protein was used as the immunogen for the ATP5O antibody.

Storage

Aliquot the ATP5O antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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