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Home >> Antibodies >> ATP5H Antibody

ATP5H Antibody (R31741)

  Catalog No Formulation Size Price (USD)  
Image R31741 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 329
Western blot testing of ATP5H antibody and Lane 1: rat brain; 2: mouse brain; 3: human placenta; 4: (h) HeLa lysate. Expected/observed molecular weight: ~22kDa.
IHC-P: ATP5H antibody testing of human breast cancer tissue. HIER: steamed with pH6 citrate buffer.
ICC testing of ATP5H antibody and A549 cells
IHC-P testing of mouse intestine tissue. HIER: steamed with pH6 citrate buffer.
IHC-P testing of rat intestine tissue. HIER: steamed with pH6 citrate buffer.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Gene ID 10476
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
Immunocytochemistry : 0.5-1ug/ml
Limitations This ATP5H antibody is available for research use only.
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Description

ATP5H is the 'd' subunit of Mitochondrial ATP synthase complex which catalyzes ATP synthesis utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. The ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo has nine known subunits (a, b, c, d, e, f, g, F6 and 8). The ATP5H gene encodes the d subunit of the Fo complex.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the ATP5H antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human partial recombinant protein (AA 2-161) was used as the immunogen for this ATP5H antibody.

Storage

After reconstitution, the ATP5H antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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