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Home >> Antibodies >> ATP5F1B Antibody / ATP synthase subunit beta / ATP5B

ATP5F1B Antibody / ATP synthase subunit beta / ATP5B (RQ7218)

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  Catalog No Formulation Size Price (USD)  
Image RQ7218 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human breast cancer with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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IHC staining of FFPE human breast cancer with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian cancer with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human esophageal squamous carcinoma tissue with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human endometrial adenocarcinoma tissue with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer tissue with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE mouse heart tissue with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat heart tissue with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human ovarian cancer tissue with ATP5F1B antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
Immunofluorescent staining of FFPE human breast cancer tissue with ATP5F1B antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
Immunofluorescent staining of FFPE human PC-3 cells with ATP5F1B antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HepG2, 2) HeLa, 3) PC-3, 4) HL60, 5) T-47D, 6) Caco-2, 7) HEL and 8) K562 cell lysate with ATP5F1B antibody. Predicted molecular weight ~56 kDa.
Western blot testing of 1) rat heart, 2) rat liver, 3) rat lung, 4) rat RH35, 5) mouse heart, 6) mouse liver, 7) mouse lung and 8) mouse RAW264.7 cell lysate with ATP5F1B antibody. Predicted molecular weight ~56 kDa.
Flow cytometry testing of human 293T cells with ATP5F1B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATP5F1B antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P06576
Localization Cytoplasmic (mitochondria)
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This ATP5F1B antibody is available for research use only.
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Description

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.

Application Notes

Optimal dilution of the ATP5F1B antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids Q123-S529) was used as the immunogen for the ATP5F1B antibody.

Storage

After reconstitution, the ATP5F1B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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