ATP5A1 Antibody / ATP5F1A / ATP synthase subunit alpha (RQ7217)

Immunofluorescent staining of FFPE human placental tissue with ATP5A1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.

Immunofluorescent staining of FFPE human colon cancer tissue with ATP5A1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.

Immunofluorescent staining of FFPE human HepG2 cells with ATP5A1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

IHC staining of FFPE human endometrial adenocarcinoma tissue with ATP5A1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human thyroid cancer tissue with ATP5A1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human rectal cancer tissue with ATP5A1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human placental tissue with ATP5A1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of human 1) SK-OV-3, 2) MCF7, 3) HL60, 4) RT4, 5) Daudi, 6) ThP-1 and 7) HepG2 cell lysate with ATP5A1 antibody. Predicted molecular weight ~54-60 kDa (multiple isoforms).

Western blot testing of 1) rat heart, 2) rat brain, 3) rat lung, 4) rat H9C2(2-1), 5) mouse heart, 6) mouse brain, 7) mouse lung and 8) mouse NIH 3T3 cell lysate with ATP5A1 antibody. Predicted molecular weight ~54-60 kDa (multiple isoforms).

Flow cytometry testing of human U937 cells with ATP5A1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATP5A1 antibody.