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Home >> Antibodies >> ATP4B Antibody / Gastric hydrogen potassium ATPase beta

ATP4B Antibody / Gastric hydrogen potassium ATPase beta (R32734)

  Catalog No Formulation Size Price (USD)  
Image R32734 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 329
Western blot testing of 1) rat stomach, 2) mouse stomach and 3) human SGC-7901 (human gastric cancer) lysate with ATP4B antibody at 0.5ug/ml. Predicted molecular weight ~34 kDa (core peptide), ~52 kDa (beta subunit precursor), 60-80 kDa (glycosylated form).
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
UniProt P51164
Applications Western Blot : 0.5-1ug/ml
Limitations This ATP4B antibody is available for research use only.
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Description

This gene encodes the beta subunit of the gastric H+, K+-ATPase. The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion.

Application Notes

Optimal dilution of the ATP4B antibody should be determined by the researcher.

Immunogen

Amino acids 1-28 (MAALQEKKTCGQRMEEFQRYCWNPDTGQ) from the human protein were used as the immunogen for the ATP4B antibody.

Storage

After reconstitution, the ATP4B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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