ATP4A Antibody / Potassium-transporting ATPase alpha chain 1 (RQ7146)

Description

ATP4A, also known as the potassium-transporting ATPase alpha chain 1, is the catalytic subunit of the gastric H⁺/K⁺-ATPase pump. This membrane-bound enzyme is responsible for exchanging cytoplasmic protons for extracellular potassium ions, a process essential for gastric acid secretion in the stomach's parietal cells. The alpha subunit contains the ion transport sites, ATP-binding regions, and phosphorylation domains that drive the pump's electrogenic activity.

By maintaining the acidic environment of the stomach, ATP4A plays a key role in digestion and the activation of gastric enzymes. Its expression and function are also studied in the context of ion transport regulation, membrane protein trafficking, and pharmacologic inhibition mechanisms.

The ATP4A antibody is a dependable reagent for detecting endogenous ATP4A in applications such as western blot, immunohistochemistry, and immunofluorescence. Researchers use the ATP4A antibody from NSJ Bioreagents to measure protein abundance, assess membrane localization, and monitor changes in expression under defined experimental conditions. With high specificity and consistent performance, the ATP4A antibody supports detailed investigations into ion transport biology, membrane protein regulation, and gastric physiology.

Application Notes

Optimal dilution of the ATP4A antibody should be determined by the researcher.

Immunogen

Amino acids AELEQKYQTSATKGLSASLAAELLLRD were used as the immunogen for the ATP4A antibody.

Storage

After reconstitution, the ATP4A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.