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Home >> Antibodies >> ATP Citrate Lyase Antibody / ACL

ATP Citrate Lyase Antibody / ACL (R32492)

  Catalog No Formulation Size Price (USD)  
Image R32492 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 329
Western blot testing of human MCF7 cell lysate with ATP Citrate Lyase antibody at 0.5ug/ml. Predicted molecular weight ~121 kDa.
IHC testing of FFPE human intestinal cancer tissue with ATP Citrate Lyase antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.
IHC testing of FFPE rat pancreas with ATP Citrate Lyase antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.
Flow cytometry testing of human A549 cells with ATP Citrate Lyase antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=ATP Citrate Lyase antibody.
Flow cytometry testing of human U-87 MG cells with ATP Citrate Lyase antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=ATP Citrate Lyase antibody.
Flow cytometry testing of human U-2 OS cells with ATP Citrate Lyase antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=ATP Citrate Lyase antibody.
ICC staining of FFPE human A431 cells with ATP Citrate Lyase antibody (red) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Availability 1-3 business days
Species Reactivity Human, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P53396
Localization Cytoplasmic, nuclear
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Immunocytochemistry (FFPE) : 2-4ug/ml
Flow cytometry : 1-3ug/10^6 cells
Limitations This ATP Citrate Lyase antibody is available for research use only.
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Description

ATP citrate lyase, aslo known as ACLY, is an enzyme that in animals represents an important step in fatty acid biosynthesis. ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of apparently identical subunits. The product, acetyl-CoA, in animals serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. It is activated by insulin. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. In plants, ATP citrate lyase generates the acetyl-CoA for cytosolically-synthesized metabolites.

Application Notes

Differences in protocols and secondary/substrate sensitivity may require the ATP Citrate Lyase antibody to be titrated for optimal performance.

Immunogen

Amino acids M1-I180 from the human protein were used as the immunogen for the ATP Citrate Lyase antibody.

Storage

After reconstitution, the ATP Citrate Lyase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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