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Home >> Antibodies >> ATIC Antibody / AICAR transformylase / PURH

ATIC Antibody / AICAR transformylase / PURH (RQ7964)

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  Catalog No Formulation Size Price (USD)  
Image RQ7964 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) rat stomach, 2) rat kidney, 3) rat testis, 4) mouse kidney and 5) mouse testis tissue lysate with ATIC antibody. Predicted molecular weight ~65 kDa.
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Western blot testing of 1) rat stomach, 2) rat kidney, 3) rat testis, 4) mouse kidney and 5) mouse testis tissue lysate with ATIC antibody. Predicted molecular weight ~65 kDa.
Western blot testing of human 1) Caco-2, 2) HepG2, 3) K562, 4) HEK293 and 5) HeLa cell lysate with ATIC antibody. Predicted molecular weight ~65 kDa.
Flow cytometry testing of human K562 cells with ATIC antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATIC antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P31939
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This ATIC antibody is available for research use only.
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Description

Bifunctional purine biosynthesis protein PURH is a protein that in humans is encoded by the ATIC gene. This gene encodes a bifunctional protein that catalyzes the last two steps of the de novo purine biosynthetic pathway. The N-terminal domain has phosphoribosylaminoimidazolecarboxamide formyltransferase activity, and the C-terminal domain has IMP cyclohydrolase activity. A mutation in this gene results in AICA-ribosiduria.

Application Notes

Optimal dilution of the ATIC antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids T37-H592) was used as the immunogen for the ATIC antibody.

Storage

After reconstitution, the ATIC antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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