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- Email: info@nsjbio.com
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Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Apg9 plays a direct role in the formation of the cytoplasm to vacuole targeting and autophagic vesicles, possibly serving as a marker for a specialized compartment essential for these vesicle-mediated alternative targeting pathways.
The stated application concentrations are suggested starting points. Titration of the ATG9A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A portion of amino acids 717-746 from the human protein was used as the immunogen for the ATG9A antibody.
Aliquot the ATG9A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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