• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Home >> Antibodies >> ATG7 Antibody

ATG7 Antibody (RQ8481)

  Catalog No Formulation Size Price (USD)  
Image RQ8481 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
Bulk quote request
IHC staining of FFPE human liver cancer tissue with ATG7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian serous adenocarcinoma tissue with ATG7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with ATG7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung adenocarcinoma tissue with ATG7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human meningioma tissue with ATG7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human HeLa cells with ATG7 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HepG2 and 2) PC-3 cell lysate with ATG7 antibody. Predicted molecular weight: 70-80 kDa.
Flow cytometry testing of fixed and permeabilized human HepG2 cells with ATG7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATG7 antibody.
Availability 1-3 days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt O95352
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
ELISA : 0.1-0.5ug/ml
Limitations This ATG7 antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

  • Applications : WB, ELISA
    Reactivity : Human
  • Applications : WB
    Reactivity : Human, Mouse, Rat
    Rab Mono Image
  • Applications : WB
    Reactivity : Human
    Rab Mono Image
  • Applications : WB, FACS
    Reactivity : Human, Mouse, Rat
  • Applications : WB, IHC, ELISA
    Reactivity : Human, Mouse
    Pred. Reactivity : Rat
  • Applications : WB, IHC, ELISA
    Reactivity : Human, Mouse
    Pred. Reactivity : Chicken, Rat
  • Applications : IF, WB, IHC, ELISA
    Reactivity : Human, Mouse
    Pred. Reactivity : Rat
  • Applications : WB
    Reactivity : Human, Mouse, Rat
  • Applications : WB, FACS, Direct ELISA
    Reactivity : Human
  • Applications : WB, IHC-P, FACS
    Reactivity : Human, Mouse, Rat
  • Applications : IF, WB, IHC-P
    Reactivity : Human, Mouse

Description

Autophagy related 7 is a protein in humans encoded by ATG7 gene. This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants.

Application Notes

Optimal dilution of the ATG7 antibody should be determined by the researcher.

Immunogen

An E.coli-derived human recombinant protein (amino acids D71-K645) was used as the immunogen for the ATG7 antibody.

Storage

After reconstitution, the ATG7 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.