ATG7 Antibody / Autophagy Related Protein 7 Antibody (RQ8481)

ATG7 Antibody Human Liver Cancer IHC. Immunohistochemistry was performed on FFPE human liver cancer tissue using ATG7 Antibody. Heat-induced epitope retrieval was carried out by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to incubation with the primary antibody. Bound antibody was visualized using an HRP-conjugated secondary antibody and DAB chromogen. Strong cytoplasmic staining is observed throughout the liver carcinoma cells, while the surrounding stromal tissue exhibits comparatively low background staining. These results demonstrate that this Autophagy Related Protein 7 Antibody is suitable for immunohistochemical detection of ATG7 in formalin-fixed, paraffin-embedded human liver cancer tissue.

ATG7 Antibody Human Ovarian Serous Adenocarcinoma IHC. Immunohistochemistry was performed on FFPE human ovarian serous adenocarcinoma tissue using ATG7 Antibody. Heat-induced epitope retrieval was carried out by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to incubation with the primary antibody. Bound antibody was visualized using an HRP-conjugated secondary antibody and DAB chromogen. Moderate to strong cytoplasmic staining is observed throughout the ovarian serous adenocarcinoma cells, while the surrounding stromal tissue exhibits comparatively low background staining. These results demonstrate that this Autophagy Related Protein 7 Antibody is suitable for immunohistochemical detection of ATG7 in formalin-fixed, paraffin-embedded human ovarian serous adenocarcinoma tissue.

ATG7 Antibody Human Colorectal Adenocarcinoma IHC. Immunohistochemistry was performed on FFPE human colorectal adenocarcinoma tissue using ATG7 Antibody. Heat-induced epitope retrieval was carried out by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to incubation with the primary antibody. Bound antibody was visualized using an HRP-conjugated secondary antibody and DAB chromogen. Moderate cytoplasmic staining is observed throughout the colorectal adenocarcinoma cells, while the surrounding stromal tissue exhibits comparatively low background staining. These results demonstrate that this Autophagy Related Protein 7 Antibody is suitable for immunohistochemical detection of ATG7 in formalin-fixed, paraffin-embedded human colorectal adenocarcinoma tissue.

ATG7 Antibody Human Lung Adenocarcinoma IHC. Immunohistochemistry was performed on FFPE human lung adenocarcinoma tissue using ATG7 Antibody. Heat-induced epitope retrieval was carried out by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to incubation with the primary antibody. Bound antibody was visualized using an HRP-conjugated secondary antibody and DAB chromogen. Moderate to strong cytoplasmic staining is observed throughout the lung adenocarcinoma cells, while the surrounding stromal tissue exhibits comparatively low background staining. These results demonstrate that this Autophagy Related Protein 7 Antibody is suitable for immunohistochemical detection of ATG7 in formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue.

ATG7 Antibody Human Meningioma IHC. Immunohistochemistry was performed on FFPE human meningioma tissue using ATG7 Antibody. Heat-induced epitope retrieval was carried out by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to incubation with the primary antibody. Bound antibody was visualized using an HRP-conjugated secondary antibody and DAB chromogen. Moderate cytoplasmic staining is observed throughout the meningioma cells, while the surrounding stromal tissue exhibits comparatively low background staining. These results demonstrate that this Autophagy Related Protein 7 Antibody is suitable for immunohistochemical detection of ATG7 in formalin-fixed, paraffin-embedded human meningioma tissue.

ATG7 Antibody HeLa Cell IF. Immunofluorescence staining was performed on FFPE human HeLa cells using ATG7 Antibody. Heat-induced epitope retrieval was carried out by steaming tissue sections in pH 6 citrate buffer for 20 minutes prior to incubation with the primary antibody. Bound antibody was detected with a Fluoro488-conjugated secondary antibody (green), and nuclei were counterstained with DAPI (blue). Diffuse cytoplasmic staining is observed throughout the HeLa cells with minimal nuclear signal, consistent with the expected intracellular localization of ATG7. These results demonstrate that this Autophagy Related Protein 7 Antibody is suitable for immunofluorescent detection of ATG7 in FFPE human cells.

ATG7 Antibody Human Cancer Cell WB. Western blot analysis was performed using ATG7 Antibody on human HepG2 (lane 1) and PC-3 (lane 2) cell lysates. A specific immunoreactive band is detected at approximately 70-80 kDa in both cell lines, consistent with the expected molecular weight of ATG7. Comparable protein expression is observed in both human cancer cell lysates with minimal background staining. These results demonstrate that this Autophagy Related Protein 7 Antibody is suitable for Western blot detection of ATG7 in human cell lysates.

ATG7 Antibody HepG2 Cell FACS. Flow cytometric analysis was performed on fixed and permeabilized human HepG2 cells using ATG7 Antibody at 1 ug/10^6 cells following blocking with normal goat serum. The red histogram represents unstained cells, the green histogram represents the isotype control, and the blue histogram represents cells stained with the ATG7 antibody. A clear rightward shift of the ATG7-stained population relative to both control populations demonstrates specific intracellular antibody binding and confirms ATG7 expression in HepG2 cells. These results demonstrate that this Autophagy Related Protein 7 Antibody is suitable for flow cytometric detection of ATG7.