ATG7 Antibody / Autophagy Flux Antibody (F54380)

ATG7 Antibody Autophagy Flux IF. Immunofluorescence staining was performed on untreated and chloroquine-treated (50 uM, 16 hours) human U-251 cells using ATG7 Antibody (green) with DAPI nuclear counterstain (blue). Untreated cells display predominantly diffuse cytoplasmic staining, whereas chloroquine treatment results in prominent punctate ATG7 staining throughout the cytoplasm, consistent with accumulation of autophagy-related structures following inhibition of autophagic flux. These results demonstrate that this Autophagy Flux Antibody is suitable for monitoring ATG7 localization during pharmacological modulation of autophagy.

ATG7 Antibody Human Liver IHC. Immunohistochemistry was performed on FFPE human liver tissue using ATG7 Antibody. Heat-induced epitope retrieval was performed by steaming tissue sections in pH 9 EDTA buffer for 20 minutes followed by cooling prior to incubation with the primary antibody. Bound antibody was visualized using an HRP-conjugated secondary antibody and DAB chromogen. Strong cytoplasmic staining is observed throughout hepatocytes with minimal background staining, consistent with endogenous ATG7 expression in liver tissue. These results demonstrate that this Autophagy Flux Antibody is suitable for immunohistochemical detection of ATG7 in formalin-fixed, paraffin-embedded human liver tissue.

Western blot testing of human 1) Caki-1, 2) HeLa and 3) HepG2 cell lysate with ATG7 antibody. Predicted molecular weight: 70-80 kDa.

Western blot testing of human HEK293 cell lysate with ATG7 antibody. Predicted molecular weight: 70-80 kDa.

Western blot testing of 1) human HepG2 and 2) mouse NIH 3T3 cell lysate with ATG7 antibody. Predicted molecular weight: 70-80 kDa.