ATG7 Antibody / Knockout Validated Antibody [clone ACHA-1] (RQ4805)

ATG7 Antibody Knockout Validated WB. Western blot analysis was performed using ATG7 Antibody at 0.5 ug/ml. Lane 1: human HeLa wild-type whole cell lysate; lane 2: human HeLa ATG7 knockout whole cell lysate. Proteins were separated on a 10% SDS-PAGE gel, transferred to a nitrocellulose membrane, and the blot was incubated with the primary antibody overnight at 4°C. Bound antibody was detected using an HRP-conjugated goat anti-rabbit IgG secondary antibody and enhanced chemiluminescence. A specific immunoreactive band is detected at approximately 78 kDa in the wild-type lysate but is absent in the ATG7 knockout lysate, while ACTB serves as the loading control. These results demonstrate that this Knockout Validated Antibody specifically recognizes ATG7 and provides high-confidence target detection by Western blot.

ATG7 Antibody HeLa Cell IF. Immunofluorescence analysis was performed on human HeLa cells using ATG7 Antibody at a 1:50 dilution. Bound antibody was detected with a green fluorescent secondary antibody, while F-actin was counterstained in red and nuclei were counterstained in blue. The merged image demonstrates diffuse cytoplasmic ATG7 staining with minimal nuclear signal, consistent with the expected intracellular localization of ATG7. These results demonstrate that this Knockout Validated Antibody is suitable for immunofluorescent detection of ATG7 in cultured human cells.

ATG7 Antibody Human, Mouse and Rat WB. Western blot analysis was performed using ATG7 Antibody. Lane 1: human HepG2 cell lysate; lane 2: mouse spleen lysate; lane 3: rat kidney lysate. A specific immunoreactive band is detected at approximately 78 kDa in all three samples, consistent with the expected molecular weight of ATG7. Comparable expression is observed in human, mouse, and rat lysates with minimal background staining. These results demonstrate that this Knockout Validated Antibody is suitable for Western blot detection of ATG7 across multiple species.

ATG7 Antibody Human Cell Line WB. Western blot analysis was performed using ATG7 Antibody. Lane 1: human HeLa whole cell lysate; lane 2: human HeLa whole cell lysate; lane 3: human HeLa whole cell lysate; lane 4: human SiHa whole cell lysate; lane 5: human HepG2 whole cell lysate; lane 6: human K562 whole cell lysate. Proteins were separated on a 5-20% SDS-PAGE gel, transferred to a nitrocellulose membrane, and the blot was incubated with the primary antibody overnight at 4°C. Bound antibody was detected using an HRP-conjugated goat anti-rabbit IgG secondary antibody and enhanced chemiluminescence. A specific immunoreactive band is detected at approximately 78 kDa in all tested cell lines, consistent with the expected molecular weight of ATG7. These results demonstrate that this Knockout Validated Antibody is suitable for Western blot detection of ATG7 in human cell lysates.

ATG7 Antibody Mouse and Rat Tissue WB. Western blot analysis was performed using ATG7 Antibody on mouse heart, mouse liver, mouse spleen, mouse lung, mouse kidney, mouse brain, mouse skin, rat heart, rat spleen, rat kidney, and rat brain tissue lysates. A prominent immunoreactive band is detected at approximately 78 kDa in all examined tissues, consistent with the expected molecular weight of ATG7. Differences in band intensity reflect tissue-specific expression levels, while additional higher or lower molecular weight bands may represent isoforms, post-translationally modified species, or nonspecific reactivity. These results demonstrate that this Knockout Validated Antibody is suitable for Western blot detection of ATG7 in mouse and rat tissues.