ATG5 Antibody [clone ATG5/2101] (V3951)

IHC staining of FFPE human endometrium with ATG5 antibody (clone ATG5/2101). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

IHC staining of FFPE human brain with ATG5 antibody (clone ATG5/2101). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human THP1 and human brain lysate with ATG5 antibody (clone ATG5/2101). Expected molecular weight ATG5: ~32 kDa; ATG5/ATG12 heterodimer: ~56 kDa.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ATG5 antibody (clone ATG5/2101). These results demonstrate the foremost specificity of the ATG5/2101 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free ATG5 antibody (clone ATG5/2101) as confirmation of integrity and purity.