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Home >> Antibodies >> ATG5 Antibody

ATG5 Antibody [clone ATG5/2553] (V8240)

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  Catalog No Formulation Size Price (USD)  
Image V8240-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8240-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8240SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human uterus with ATG5 antibody (clone ATG5/2553). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
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IHC staining of FFPE human uterus with ATG5 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human endometrium with ATG5 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human brain with ATG5 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Western blot testing of human K562, U-87 and A431 cell lysate with ATG5 antibody. Predicted molecular weight ATG5: ~32 kDa; ATG5/ATG12 heterodimer: ~56 kDa.
Western blot testing of human ThP1 and brain lysate with ATG5 antibody. Predicted molecular weight ATG5: ~32 kDa; ATG5/ATG12 heterodimer: ~56 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name ATG5/2553
Purity Protein G affinity chromatography
UniProt Q9H1Y0
Localization Cytoplasmic
Applications ELISA (order BSA-free format for coating) :
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This ATG5 antibody is available for research use only.
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Description

The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. The ATG5 protein is essential for autophagy; a process that is usually beneficial for cells to self-degrade their own components when they are no longer useful.

Application Notes

Optimal dilution of the ATG5 antibody should be determined by the researcher.

Immunogen

A recombinant human partial protein (amino acids 1-119) was used as the immunogen for the ATG5 antibody.

Storage

Store the ATG5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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