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Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. [UniProt]
Optimal dilution of the ATG12 antibody should be determined by the researcher.
A synthetic peptide specific to human ATG12 was used as the immunogen for the ATG12 antibody.
Store the ATG12 antibody at -20oC.
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