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Home >> Antibodies >> ATG12 Antibody

ATG12 Antibody [clone 43CT73.3.5.5.4] (F40210)

  Catalog No Formulation Size Price (USD)  
Image F40210-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 329
Image
F40210-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 135
Western blot analysis of ATG12 antibody and recombinant protein.
IHC analysis of FFPE human hepatocarcinoma tissue and lung carcinoma tissue stained with ATG12 antibody
IHC analysis of FFPE human lung carcinoma tissue stained with ATG12 antibody
ATG12 antibody tested in 293 cells, fixed in PFA permeablilized with 0.2% Saponin, blocked with 10% goat serum. Secondary was mouse 555. The right is in full medium (FM) and the left with EBSS (no Leupeptin) (Provided by Nicole McKnight & Sharon Tooze).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, k
Clone Name 43CT73.3.5.5.4
Purity Purified
UniProt O94817
Applications Western blot : 1:200-1:2000
IHC (Paraffin) : 1:50-1:200
Immunofluorescence : 1:10-1:50
Limitations This ATG12 antibody is available for research use only.
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Description

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy.

Application Notes

Titration of the ATG12 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

APG12L recombinant protein was used to produce this monoclonal antibody.

Storage

Aliquot the ATG12 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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