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Home >> Antibodies >> Ataxin 3 Antibody [Discontinued, view alternative antibodies]

Ataxin 3 Antibody [Discontinued, view alternative antibodies] (R30944)

  Catalog No Formulation Size Price (USD)  
Image R30944 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 299
Western blot testing of Ataxin 3 antibody and rat samples 1: brain; 2: heart; 3: placenta; and human samples 4: HeLa; 5: PANC; 6: SMMC-7721; 7: COLO320; 8: MCF-7 cell lysate. Predicted molecular weight ~41 kDa.
IHC-P: Ataxin 3 antibody testing of human lung cancer tissue. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human SiHa cells with Ataxin 3 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Ataxin 3 antibody.
Flow cytometry testing of human A549 cells with Ataxin 3 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Ataxin 3 antibody.
Flow cytometry testing of human MCF7 cells with Ataxin 3 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Ataxin 3 antibody.
Availability Discontinued
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P54252
Localization Nuclear and cytoplasmic
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
Flow Cytometry : 1-3ug/10^6 cells
Limitations This Ataxin 3 antibody is available for research use only.
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Description

Ataxin 3 is a protein that in humans is encoded by the ATXN3 gene. ATXN3 ranges in size from 360 to 374 amino acids. Using Northern blot analysis showed that ATXN3 mRNA was ubiquitously expressed in human tissues. There are at least 4 ATXN3 transcripts of 1.4, 1.8, 4.5, and 7.5 kb and suggested that the different mRNA species probably result from differential splicing and polyadenylation. Machado-Joseph disease, also known as spinocerebellar ataxia-3, is an autosomal dominant neurologic disorder. The protein encoded by the ATXN3 gene contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is the cause of Machado-Joseph disease. There is an inverse correlation between the age of onset and CAG repeat numbers. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. Ataxin-3 interacted with two human homologs of the yeast DNA repair protein RAD23, HHR23A (RAD23A) and HHR23B (RAD23B). Both normal and mutant ataxin-3 proteins interacted with the ubiquitin-like domain at the N terminus of the HHR23 proteins, which is a motif important for nucleotide excision repair. However, in HEK 293 cells, HHR23A was recruited to intranuclear inclusions formed by the mutant ATXN3 through its interaction with ATXN3.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the Ataxin 3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the N-terminus of human Ataxin 3 (EKQEGSLCAQHCLNN) was used as the immunogen for this Ataxin 3 antibody (100% homologous in human, mouse and rat).

Storage

After reconstitution, the Ataxin 3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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