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Home >> Antibodies >> Ataxin-2 Antibody

Ataxin-2 Antibody (R31210)

  Catalog No Formulation Size Price (USD)  
Image R31210 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 329
Western blot testing of Ataxin-2 antibody and human lysate samples: 1) PANC, 2) SMMC-7721, 3) HeLa. Expected molecular weight: 114~140 kDa.
IF/ICC testing of human A549 cells with Ataxin-2 antibody at 2ug/ml.
IF/ICC testing of human A549 cells with Ataxin-2 antibody at 2ug/ml.
IF/ICC testing of human A549 cells with Ataxin-2 antibody at 2ug/ml.
Flow cytometry testing of human A549 cells with Ataxin-2 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Ataxin-2 antibody.
Flow cytometry testing of human A431 cells with Ataxin-2 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Ataxin-2 antibody.
Flow cytometry testing of human K562 cells with Ataxin-2 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Ataxin-2 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt Q99700
Applications Western blot : 0.5-1ug/ml
Flow Cytometry : 1-3ug/10^6 cells
IF/ICC : 2-3ug/ml
Limitations This Ataxin-2 antibody is available for research use only.
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Description

Ataxin-2, the protein encoded by the ATXN2 gene, contains a polyglutamine tract, long expansions (greater than 33 repeats) of which result in spinocerebellar ataxia-2 (SCA2), an autosomal dominant form of olivopontocerebellar atrophy. The gene for spinocerebellar ataxia type 2 (SCA2) has been mapped to 12q24.1. Ataxin-2 associates with L- and T-plastin and overexpression leads to accumulation of T-plastin in mammalian cells.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the Ataxin-2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the C-terminus of human Ataxin-2 (TTAHFPYMTHPSVQAHHQQQL) was used as the immunogen for this Ataxin-2 antibody (100% mouse homology).

Storage

After reconstitution, the Ataxin-2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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