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Home >> Antibodies >> Aspartoacylase Antibody / ASPA

Aspartoacylase Antibody / ASPA (F54432)

  Catalog No Formulation Size Price (USD)  
Image F54432-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 359
Image
F54432-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 149
IHC testing of FFPE human skeletal muscle tissue with Aspartoacylase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human MCF7 cells with Aspartoacylase antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human MCF7 cell lysate with Aspartoacylase antibody. Predicted molecular weight ~36 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Aspartoacylase antibody.
Flow cytometry testing of human MDA-MB-468 cells with Aspartoacylase antibody; Blue=isotype control, Green= Aspartoacylase antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity SAS precipitation
UniProt P45381
Localization Nuclear, cytoplasmic
Applications Immunofluorescence : 1:25
Western blot : 1:500-1:2000
Flow cytometry : 1:25 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:25
Limitations This Aspartoacylase antibody is available for research use only.
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Description

ASPA is an enzyme that catalyzes the conversion of N-acetyl_L-aspartic acid (NAA) to aspartate and acetate. NAA is abundant in the brain where hydrolysis by aspartoacylase is thought to help maintain white matter. This protein is an NAA scavenger in other tissues.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Aspartoacylase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 82-110 from the human protein was used as the immunogen for the Aspartoacylase antibody.

Storage

Aliquot the Aspartoacylase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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