ASGP-2 Antibody | MUC4 [clone MUC4/2863] (V5951)

Analysis of Protein Array containing more than 19,000 full-length human proteins using ASGP-2/MUC4 antibody (clone MUC4/2863). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

SDS-PAGE analysis of purified ASGP-2/MUC4 antibody (clone MUC4/2863). Confirmation of Purity and Integrity of Antibody.

Immunohistochemistry analysis of ASGP-2 / MUC4 antibody (clone MUC4/2863) in human stomach tissue. Formalin-fixed, paraffin-embedded human stomach section shows membranous and apical cytoplasmic brown chromogenic staining in gastric epithelial cells lining glandular structures, consistent with MUC4 expression, while stromal cells exhibit minimal staining and nuclei appear blue. The inset shows PBS used in place of primary antibody as a negative control with no specific staining observed. Heat-induced epitope retrieval was performed by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95oC followed by cooling at room temperature for 20 minutes prior to staining.