- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Aryl hydrocarbon Receptor Antibody / AHR (R30876)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
R30876 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 439 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse, Rat |
| Format | Antigen affinity purified |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Antigen affinity |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | P35869 |
| Localization | Cytoplasmic, nuclear |
| Applications | Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Immunofluorescence : 5ug/ml Flow Cytometry : 1-3ug/million cells |
| Limitations | This Aryl hydrocarbon Receptor antibody is available for research use only. |
|
Review this product on BioCompare and get a $20 Amazon gift card
|
Related Products
|
Aryl Hydrocarbon Receptor antibody detects the AHR protein, a ligand-activated transcription factor belonging to the basic helix-loop-helix Per-ARNT-Sim (bHLH-PAS) family. The UniProt recommended name is Aryl hydrocarbon receptor (AHR). This cytoplasmic receptor mediates the cellular response to environmental toxins, xenobiotics, and endogenous metabolites, playing an essential role in detoxification, immune regulation, and development.
Functionally, Aryl Hydrocarbon Receptor antibody identifies an 848-amino-acid cytoplasmic protein that remains inactive when bound to chaperones such as HSP90, XAP2, and p23. Upon ligand binding, AHR translocates to the nucleus, where it dimerizes with the Aryl hydrocarbon receptor nuclear translocator (ARNT). This complex binds to xenobiotic response elements (XREs) in target gene promoters, activating transcription of detoxification enzymes such as CYP1A1, CYP1A2, and CYP1B1.
The AHR gene is located on chromosome 7p21.1 and is broadly expressed across multiple tissues, including liver, lung, thymus, and skin. AHR serves as a molecular sensor that integrates environmental and metabolic signals. Its canonical pathway involves regulation of phase I and phase II xenobiotic-metabolizing enzymes, supporting the detoxification of aromatic hydrocarbons and halogenated compounds. Noncanonical AHR signaling also influences immune cell differentiation, circadian rhythm, and stem cell function.
Physiologically, AHR modulates differentiation of regulatory T cells and Th17 cells, linking environmental cues to immune homeostasis. Endogenous ligands, including tryptophan metabolites such as kynurenine and FICZ, activate AHR signaling in immune and barrier tissues. Beyond immune regulation, AHR plays developmental roles in vasculature formation, epidermal barrier function, and liver morphogenesis. Its expression in intestinal and pulmonary epithelia supports adaptive responses to microbial metabolites and xenobiotic exposure.
Dysregulated AHR signaling contributes to diverse pathologies. Persistent activation by environmental toxins like dioxins (e.g., TCDD) leads to toxic responses and carcinogenesis, while insufficient AHR signaling impairs mucosal immunity and epithelial regeneration. In cancer biology, AHR acts context-dependently as either an oncogene or tumor suppressor, influencing cell cycle control, invasion, and immune evasion. In metabolic and neuroimmune systems, AHR coordinates detoxification with inflammatory and oxidative stress responses.
Aryl Hydrocarbon Receptor antibody is widely used in toxicology, immunology, and molecular signaling research. It is suitable for western blotting, immunohistochemistry, and immunofluorescence to detect AHR localization and activation states. This antibody supports studies of xenobiotic metabolism, transcriptional regulation, and ligand-dependent signaling. In translational research, AHR detection aids in understanding how environmental exposures and endogenous ligands shape immunity and cancer biology.
Structurally, AHR contains an N-terminal bHLH DNA-binding region, tandem PAS domains for ligand and ARNT interaction, and a C-terminal transactivation domain. Ligand binding triggers conformational rearrangement and nuclear translocation. NSJ Bioreagents provides Aryl Hydrocarbon Receptor antibody reagents validated for use in environmental toxicology, immune modulation, and transcriptional pathway research.
The stated application concentrations are suggested starting amounts. Titration of the Aryl hydrocarbon Receptor antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Amino acids 832-848 (HPSEARPFPDLTSSGFL-human) were used as the immunogen for this Aryl hydrocarbon Receptor antibody.
After reconstitution, the Aryl hydrocarbon Receptor antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Your bulk quote request has been submitted successfully!
Please contact us if you have any questions.
Powered by Bioz
