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Home >> Antibodies >> ARPC1B Antibody

ARPC1B Antibody (RQ4972)

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Image RQ4972 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) human placenta, 2) human PC-3, 3) human U-937, 4) rat spleen and 5) mouse spleen with ARPC1B antibody. Predicted molecular weight ~41 kDa.
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Western blot testing of 1) human placenta, 2) human PC-3, 3) human U-937, 4) rat spleen and 5) mouse spleen with ARPC1B antibody. Predicted molecular weight ~41 kDa.
IHC staining of FFPE human tonsil with ARPC1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE mouse spleen with ARPC1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat spleen with ARPC1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Flow cytometry testing of human PC-3 cells with ARPC1B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ARPC1B antibody.
Flow cytometry testing of human Caco-2 cells with ARPC1B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ARPC1B antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt O15143
Applications Western blot : 0.1-0.5ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This ARPC1B antibody is available for research use only.
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Description

Actin-related protein 2/3 complex subunit 1B is a protein that in humans is encoded by the ARPC1B gene. It is mapped to 7q22.1. This gene encodes one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1A. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages. This protein also has a role in centrosomal homeostasis by being an activator and substrate of the Aurora A kinase.

Application Notes

Optimal dilution of the ARPC1B antibody should be determined by the researcher.

Immunogen

Amino acids Q293-K372 from the human protein were used as the immunogen for the ARPC1B antibody.

Storage

Store the ARPC1B antibody at -20oC.

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