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Home >> Antibodies >> ARPC1B Antibody

ARPC1B Antibody (F54999)

  Catalog No Formulation Size Price (USD)  
Image F54999-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 369
Image
F54999-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 155
IHC testing of FFPE human lung carcinoma tissue with ARPC1B antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human HeLa cell lysate with ARPC1B antibody. Predicted molecular weight ~41 kDa.
Western blot testing of human A431 cell lysate with ARPC1B antibody. Predicted molecular weight ~41 kDa.
Flow cytometry testing of human MCF7 cells with ARPC1B antibody; Blue=isotype control, Green= ARPC1B antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt O15143
Localization Cytoplasmic, nuclear
Applications Western blot : 1:500-1:1000
Flow cytometry : 1:10-1:50 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:50-1:100
Limitations This ARPC1B antibody is available for research use only.
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Description

ARPC1B is one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1A. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex.

Application Notes

The stated application concentrations are suggested starting points. Titration of the ARPC1B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 159-188 from the human protein was used as the immunogen for the ARPC1B antibody.

Storage

Aliquot the ARPC1B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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