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Home >> Antibodies >> Aromatase Antibody / CYP19A1

Aromatase Antibody / CYP19A1 (RQ6861)

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  Catalog No Formulation Size Price (USD)  
Image RQ6861 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) rat testis, 2) rat ovary, 3) rat brain, 4) rat C6, 5) mouse testis, 6) mouse ovary, 7) mouse brain and 8) mouse NIH 3T3 cell lysate with Aromatase antibody. Predicted molecular weight ~58 kDa.
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Western blot testing of 1) rat testis, 2) rat ovary, 3) rat brain, 4) rat C6, 5) mouse testis, 6) mouse ovary, 7) mouse brain and 8) mouse NIH 3T3 cell lysate with Aromatase antibody. Predicted molecular weight ~58 kDa.
Western blot testing of human 1) placenta, 2) HepG2, 3) HCCT, 4) HCCP, 5) U-87 MG, 6) MCF7, 7) SK-O-V3 and 8) SiHa cell lysate with Aromatase antibody. Predicted molecular weight ~58 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P28649
Applications Western blot : 1-2ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This Aromatase antibody is available for research use only.
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Description

CYP19A1, also called Aromatase, is an enzyme responsible for a key step in the biosynthesis of estrogens. It is a member of the cytochrome P450 superfamily, which are monooxygenases that catalyze many reactions involved in steroidogenesis. In particular, aromatase is responsible for the aromatization of androgens into estrogens. The CYP19 gene spans at least 70 kb of genomic DNA and contains 10 exons. By in situ hybridization, the ARO gene is mapped to 15q21.1. The aromatase enzyme can be found in many tissues including gonads, brain, adipose tissue, placenta, blood vessels, skin, bone, and endometrium, as well as in tissue of endometriosis, uterine fibroids, breast cancer, and endometrial cancer. It is an important factor in sexual development. Some bodybuilders taking steroids also take antiaromatase supplements to prevent excess testosterone conversion into estrogens, which can cause gynecomastia.

Application Notes

Optimal dilution of the Aromatase antibody should be determined by the researcher.

Immunogen

Recombinant mouse protein (amino acids N75-Q428) was used as the immunogen for the Aromatase antibody.

Storage

After reconstitution, the Aromatase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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