AKR1C1 Antibody / Steroid Metabolism Enzyme Antibody [clone AKR1C1/9063] (V5582)

AKR1C1 Antibody Liver Tissue WB. Western blot analysis of human liver tissue lysate using AKR1C1 Antibody (clone AKR1C1/9063) detects a band at approximately 37 kDa, consistent with the predicted molecular weight of AKR1C1 / Aldo-keto reductase family 1 member C1. This band aligns with the expected size of the cytosolic enzyme and supports detection of AKR1C1 in hepatic tissue, where it functions in steroid metabolism and detoxification pathways.

AKR1C1 Antibody HepG2 Cell WB. Western blot analysis of human HepG2 cell lysate using AKR1C1 Antibody (clone AKR1C1/9063) detects a prominent band at approximately 37 kDa, consistent with the predicted molecular weight of AKR1C1 / Aldo-keto reductase family 1 member C1. The strong signal in this liver-derived cell line supports detection of AKR1C1 in hepatocyte-like models, aligning with its role in steroid metabolism and detoxification pathways.

AKR1C1 Antibody Hepatocellular Carcinoma IHC. Immunohistochemistry analysis of FFPE human hepatocellular carcinoma tissue using AKR1C1 Antibody (clone AKR1C1/9063) shows cytoplasmic staining in tumor cells, consistent with AKR1C1 / Aldo-keto reductase family 1 member C1 localization as a cytosolic enzyme. The staining is heterogeneous across the tumor field, with variable intensity reflecting differences in metabolic activity among tumor cells. Surrounding non-tumor regions display comparatively lower signal. Hematoxylin counterstain provides nuclear contrast and tissue architecture. HIER: boil FFPE tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free ARK1C1 antibody (clone AKR1C1/9063) as confirmation of integrity and purity.

AKR1C1 Antibody Multi-Species Liver WB. Western blot analysis of (1) human liver, (2) mouse liver, (3) rat liver, (4) hamster liver, (5) guinea pig liver, and (6) HepG2 cell lysates using AKR1C1 Antibody (clone AKR1C1/9063) detects a band at approximately 37 kDa, consistent with the predicted molecular weight of AKR1C1 / Aldo-keto reductase family 1 member C1. Band intensity varies across species, with stronger signal observed in guinea pig liver and HepG2 cells, reflecting differences in expression level or antibody affinity. The overall pattern supports detection of AKR1C1 in hepatic tissues and liver-derived cell models involved in steroid metabolism and detoxification pathways.