Argonaute 3 Antibody / RISC Complex and Post-Transcriptional Regulation Marker (RQ6311)

Argonaute 3 Antibody K562 WB. Western blot analysis of human K562 cell lysate using Argonaute 3 antibody detecting AGO3. A band is observed at approximately 95-100 kDa, consistent with the predicted molecular weight of AGO3. The detected signal aligns with AGO3 as a cytoplasmic component of the RISC complex involved in RNA silencing and post-transcriptional gene regulation.

Argonaute 3 Antibody Multi-Sample WB. Western blot analysis of human and rodent lysates using Argonaute 3 antibody detecting AGO3. Lane 1: human K562, Lane 2: human PC-3, Lane 3: human Caco-2, Lane 4: rat brain, Lane 5: mouse brain. A prominent band is observed at approximately 95-100 kDa across samples, consistent with the predicted molecular weight of AGO3. Additional lower molecular weight bands may represent degradation or non-specific signal. The primary banding pattern supports AGO3 expression as a component of the RISC complex involved in RNA silencing and post-transcriptional gene regulation.

Argonaute 3 Antibody HeLa IF. Immunofluorescence analysis of FFPE human HeLa cells stained with Argonaute 3 antibody detecting AGO3 (red). Predominant cytoplasmic staining with additional nucleoplasmic signal is observed, consistent with AGO3 localization in RISC-associated compartments involved in RNA silencing and post-transcriptional gene regulation. DAPI nuclear stain (blue) highlights nuclei. HIER: steam section in pH 6 citrate buffer for 20 min.

Argonaute 3 Antibody U-2 OS IF. Immunofluorescence analysis of FFPE human U-2 OS cells stained with Argonaute 3 antibody detecting AGO3 (green). Predominant cytoplasmic staining with fine granular distribution is observed, consistent with localization of AGO3 in RISC-associated ribonucleoprotein complexes involved in RNA silencing and post-transcriptional gene regulation. DAPI nuclear stain (blue) highlights nuclei. HIER: steam section in pH 6 citrate buffer for 20 min.

Argonaute 3 Antibody Cervical Carcinoma IHC. Immunohistochemistry analysis of FFPE human cervical carcinoma tissue stained with Argonaute 3 antibody detecting AGO3. Tumor cells show cytoplasmic staining with variable intensity and focal nucleoplasmic signal, consistent with AGO3 localization in RISC-associated complexes involved in RNA silencing and post-transcriptional gene regulation. The staining highlights malignant epithelial regions, while stromal components show comparatively reduced signal. Hematoxylin counterstain highlights nuclei in blue. HIER: boil tissue sections in pH 8 EDTA for 20 min and allow to cool before testing.

Argonaute 3 Antibody Rat Brain IHC. Immunohistochemistry analysis of FFPE rat brain tissue stained with Argonaute 3 antibody detecting AGO3. Neuronal and glial cells show cytoplasmic staining with scattered nucleoplasmic signal, consistent with AGO3 localization in RNA silencing and RISC-associated complexes involved in post-transcriptional gene regulation. The staining is distributed throughout the neural parenchyma, while background remains low. Hematoxylin counterstain highlights nuclei in blue. HIER: boil tissue sections in pH 8 EDTA for 20 min and allow to cool before testing.

Argonaute 3 Antibody Jurkat FACS. Flow cytometry analysis of fixed and permeabilized human Jurkat cells stained with Argonaute 3 antibody detecting AGO3. The antibody signal (blue) shows a clear rightward shift compared to cells alone (red) and isotype control (green), indicating positive intracellular detection of AGO3 following fixation and permeabilization. This pattern is consistent with AGO3 as a cytoplasmic and nucleoplasmic component of RISC-associated complexes involved in RNA silencing and post-transcriptional gene regulation.