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Home >> Antibodies >> Argonaute 1 Antibody / AGO1

Argonaute 1 Antibody / AGO1 (RQ7060)

  Catalog No Formulation Size Price (USD)  
Image RQ7060 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 419
Immunofluorescent staining of FFPE human Caco-2 cells with Argonaute 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) K562, 2) Caco-2 and 3) 293T cell lysate with Argonaute 1 antibody. Predicted molecular weight ~97 kDa.
Flow cytometry testing of human MCF7 cells with Argonaute 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Argonaute 1 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9UL18
Applications Western blot : 0.5-1 ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Argonaute 1 antibody is available for research use only.
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Description

This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon.

Application Notes

Optimal dilution of the Argonaute 1 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids K131-R171) was used as the immunogen for the Argonaute 1 antibody.

Storage

After reconstitution, the Argonaute 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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