Arginase 2 Antibody / Mitochondrial Arginase Antibody (RQ5703)

Arginase 2 Antibody Human Prostate Cancer IHC. IHC staining of FFPE human prostate cancer tissue using Arginase 2 Antibody. Heat-induced epitope retrieval was performed in EDTA buffer (pH 8.0), followed by blocking with 10% goat serum. The tissue was incubated with the antibody at 2 ug/ml overnight at 4°C, followed by peroxidase-conjugated goat anti-rabbit IgG and DAB detection. Positive ARG2 staining is observed in prostate tumor cells. This Arginase 2 Antibody, also called Mitochondrial Arginase Antibody, demonstrates ARG2 expression in human prostate cancer tissue and supports studies of mitochondrial arginine metabolism and cancer metabolic biology.

Arginase 2 Antibody Human Prostate Cancer Epithelial IHC. IHC staining of FFPE human prostate cancer tissue using Arginase 2 Antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in EDTA buffer (pH 8.0) for 20 minutes, followed by cooling prior to staining. Positive ARG2 staining is observed in the glandular tumor epithelium, with comparatively limited staining in the surrounding stroma. This Arginase 2 Antibody, also called Mitochondrial Arginase Antibody, demonstrates ARG2 expression in human prostate cancer tissue and supports studies of mitochondrial arginine metabolism and tumor biology.

Arginase 2 Antibody Human Lung Cancer IHC. IHC staining of FFPE human lung cancer tissue using Arginase 2 Antibody. Heat-induced epitope retrieval was performed in EDTA buffer (pH 8.0), followed by blocking with 10% goat serum. The tissue was incubated with the antibody at 2 ug/ml overnight at 4°C, followed by peroxidase-conjugated goat anti-rabbit IgG and DAB detection. Positive ARG2 staining is observed in tumor cells. This Arginase 2 Antibody, also called Mitochondrial Arginase Antibody, demonstrates ARG2 expression in human lung cancer tissue and supports studies of mitochondrial arginine metabolism and tumor metabolic biology.

Arginase 2 Antibody Human Renal Cancer IHC. IHC staining of FFPE human renal cancer tissue using Arginase 2 Antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in EDTA buffer (pH 8.0) for 20 minutes, followed by cooling prior to staining. Positive cytoplasmic ARG2 staining is observed in tumor cells. This Arginase 2 Antibody, also called Mitochondrial Arginase Antibody, demonstrates ARG2 expression in human renal cancer tissue and supports studies of arginine metabolism and kidney tumor biology.

Arginase 2 Antibody U2OS Cell IF. Immunofluorescent staining of human U2OS cells using Arginase 2 Antibody and an Alpha Tubulin Antibody. Cells underwent enzyme antigen retrieval for 15 minutes, were blocked with 10% goat serum and incubated with both primary antibodies at 5 ug/ml overnight at 4°C. ARG2 was visualized with Fluoro488-conjugated secondary antibody (green), while alpha tubulin was detected with Fluoro594-conjugated secondary antibody (red). This Arginase 2 Antibody, also called Mitochondrial Arginase Antibody, demonstrates ARG2 expression in human U2OS cells and supports studies of mitochondrial arginine metabolism and subcellular protein expression.

Arginase 2 Antibody Human, Mouse and Rat WB. Western blot analysis of ARG2 in human HepG2, K562, 293T and Caco-2 cells, rat kidney and colon tissues, and mouse kidney and colon tissues using Arginase 2 Antibody. Samples (30 ug/lane) were separated by 10% SDS-PAGE under reducing conditions and transferred to nitrocellulose. Lanes 1-8: HepG2, K562, 293T, Caco-2, rat kidney, rat colon, mouse kidney and mouse colon, respectively. The membrane was incubated with the antibody at 0.5 ug/ml overnight at 4°C, followed by HRP-conjugated secondary antibody and ECL detection. A specific band was detected at approximately 39 kDa, consistent with the expected molecular weight of ARG2. This Arginase 2 Antibody, also called Mitochondrial Arginase Antibody, detects ARG2 across human, mouse and rat samples for studies of mitochondrial arginine metabolism.

Arginase 2 Antibody Jurkat Cell FACS. Flow cytometry analysis of fixed and permeabilized human Jurkat cells using Arginase 2 Antibody. Cells were blocked with 10% normal goat serum and incubated with the antibody at 1 ug/million cells for 30 minutes at 20°C, followed by Fluoro488-conjugated goat anti-rabbit IgG. The histogram shows ARG2 staining (blue), isotype control (green) and unstained cells (red). A strong fluorescence shift is observed with ARG2 staining relative to the isotype control. This Arginase 2 Antibody, also called Mitochondrial Arginase Antibody, detects intracellular ARG2 in human Jurkat cells by flow cytometry for studies of mitochondrial arginine metabolism.