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Home >> Antibodies >> Arginase 1 Antibody

Arginase 1 Antibody [clone T1ARG-1] (V7111)

  Catalog No Formulation Size Price (USD)  
Image V7111-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V7111-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V7111SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V7111IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
IHC testing of FFPE human hepatocellular carcinoma and Arginase 1 antibody.
Western blot testing of 1) partial recombinant ARG1 protein and 2) human liver lysate using Arginase 1 antibody. Predicted molecular weight ~35kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG3, kappa
Clone Name T1ARG-1
Purity Protein G affinity chromatography
UniProt P05089
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 2-4ug/ml for 30 min at RT (1)
Prediluted IHC only format : incubate for 30 min at RT (2)
Limitations This Arginase 1 antibody is available for research use only.
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Description

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [RefSeq]

Application Notes

Optimal dilution of the Arginase 1 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A C-terminal recombinant protein fragment from ARG1 was used as the immunogen for the Arginase 1 antibody.

Storage

Store the Arginase 1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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