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Home >> Antibodies >> Arginase 1 Antibody

Arginase 1 Antibody (R30878)

  Catalog No Formulation Size Price (USD)  
Image R30878 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 329
Western blot testing of Arginase 1 antibody and Lane 1: rat liver ; 2: rat kidney lysate. Predicted molecular weight ~35 kDa.
Availability 1-3 business days
Species Reactivity Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P07824
Applications Western blot : 0.5-1ug/ml
Limitations This Arginase 1 antibody is available for research use only.
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Description

Arginase is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. The isoform encoded by ARG1, referred to as the liver, or A-I, isoform, contributes 98% of the arginase activity in liver but is also present in red cells. Using a rat ARG1 cDNA clone to probe a human liver cDNA library, Haraguchi et al.(1987) isolated and characterized a cDNA corresponding to the gene. By immunologic studies, 90% of the arginase in red blood cells and liver was precipitated by an Arginase 1 antibody, whereas only 50% of the arginase in kidney, brain, and the gastrointestinal tract reacted with it. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Arginase 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Amino acids 307-323 (KREGNHKPETDYLKPPK-rat) were used as the immunogen for this Arginase 1 antibody.

Storage

After reconstitution, the Arginase 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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