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Home >> Antibodies >> Arginase 1 Antibody Cocktail

Arginase 1 Antibody Cocktail [clone ARG1/1125 + ARG1/1126] (V2653)

  Catalog No Formulation Size Price (USD)  
Image V2653-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V2653-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V2653SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V2653IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
IHC: Formalin-fixed, paraffin-embedded human hepatocellular carcinoma stained with Arginase 1 antibody (ARG1/1125 + ARG1/1126).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG3, kappa
Clone Name ARG1/1125 + ARG1/1126
Purity Protein G affinity chromatography
UniProt P05089
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 2-4ug/ml for 30 min at RT
Limitations This Arginase 1 antibody cocktail is available for research use only.
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Description

Recognizes a protein of 35-38kDa, which is identified as Arginase 1 (ARG1). Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes, which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibody to ARG-1 labels hepatocytes in normal tissues and granulocytes in peripheral blood. ARG-1 is a sensitive and specific marker for identification of hepatocellular carcinoma.

Application Notes

Optimal dilution of the Arginase 1 antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A recombinant fragment from amino acids 1-150 of human ARG1 was used as the immunogen for the Arginase 1 antibody cocktail.

Storage

Store the Arginase 1 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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