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Home >> Antibodies >> ARF6 Antibody / ADP-ribosylation factor 6

ARF6 Antibody / ADP-ribosylation factor 6 (R32472)

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  Catalog No Formulation Size Price (USD)  
Image R32472 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human A431 cells with ARF6 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human A431 cells with ARF6 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human melanoma with ARF6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat heart with ARF6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of human SiHa cells with ARF6 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ARF6 antibody.
Western blot testing of 1) rat kidney, 2) mouse kidney and 3) human MCF7 lysate with ARF6 antibody at 0.5ug/ml. Predicted molecular weight ~20 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P62330
Localization Cytoplasmic, cell membrane
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/10^6 cells
Immunohistochemistry (FFPE) : 1-2ug/ml
Immunofluorescence : 2-4ug/ml
Limitations This ARF6 antibody is available for research use only.
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    Reactivity : Human, Mouse, Rat

Description

ADP-ribosylation factor 6 (ARF6) is a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7.

Application Notes

Optimal dilution of the ARF6 antibody should be determined by the researcher.

Immunogen

Amino acids G2-S175 of the human protein were used as the immunogen for the ARF6 antibody.

Storage

Prior to reconstitution, store at 4oC. After reconstitution, the ARF6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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