AQP4 Antibody / Aquaporin 4 [clone AQP4/3323] (V5750)

Western blot testing of human brain tissue lysate with AQP4 antibody (clone AQP4/3323). Observed molecular weight: 35~45 kDa depending on glycosylation level.

Flow cytometry staining of PFA-fixed human Ramos cells with AQP4 antibody (clone AQP4/3323); Red=isotype control, Blue= AQP4 antibody.

IHC staining of FFPE human small intestine tissue with AQP4 antibody (clone AQP4/3323). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using AQP4 antibody (clone AQP4/3323). These results demonstrate the foremost specificity of the AQP4/3323 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free AQP4 antibody (clone AQP4/3323) as confirmation of integrity and purity.