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Home >> Antibodies >> APRT Antibody

APRT Antibody (F54787)

  Catalog No Formulation Size Price (USD)  
Image F54787-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 379
Image
F54787-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 149
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt P07741
Applications Western blot : 1:500-1:2000
Immunofluorescence : 1:25
Flow cytometry : 1:25 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:25
Limitations This APRT antibody is available for research use only.
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Description

A conserved feature of APRT is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis.

Application Notes

The stated application concentrations are suggested starting points. Titration of the APRT antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 12-40 from the human protein was used as the immunogen for the APRT antibody.

Storage

Aliquot the APRT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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