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Home >> Antibodies >> Apoptotic protease-activating factor 1 Antibody / APAF1

Apoptotic protease-activating factor 1 Antibody / APAF1 (RQ6937)

  Catalog No Formulation Size Price (USD)  
Image RQ6937 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 419
Flow cytometry testing of human U937 cells with Apoptotic protease-activating factor 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Apoptotic protease-activating factor 1 antibody.
Western blot testing of human 1) HEL and 2) human HL-60 cell lysate with Apoptotic protease-activating factor 1 antibody. Predicted molecular weight ~140 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt O14727
Applications Western blot : 0.5-1 ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Apoptotic protease-activating factor 1 antibody is available for research use only.
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Description

Apoptotic peptidase activating factor 1, also known as APAF1, is a protein which in humans is encoded by the APAF1 gene. This gene is mapped to chromosome 12q23. It encodes a cytoplasmic protein that initiates apoptosis. And it is an essential downstream effector of p53-mediated apoptosis. This protein contains several copies of the WD40 repeat domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). In the presence of cytochrome c and dATP, APAF1 assembles into an oligomeric apoptosome, which is responsible for activation of procaspase-9 and maintenance of the enzymatic activity of processed caspase-9. Furthermore, APAF1 is inactivated in metastatic melanomas, leading to defects in the execution of apoptotic cell death. Additionally, APAF1 has been shown to interact with NLRP1, Caspase-9, APIP, BCL2-like 1 and HSPA4.

Application Notes

Optimal dilution of the Apoptotic protease-activating factor 1 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids M1-E1248) was used as the immunogen for the Apoptotic protease-activating factor 1 antibody.

Storage

After reconstitution, the Apoptotic protease-activating factor 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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