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Home >> Antibodies >> Apoptosis inhibitor 5 Antibody / API5

Apoptosis inhibitor 5 Antibody / API5 (R30177)

  Catalog No Formulation Size Price (USD)  
Image R30177 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 389
IHC staining of FFPE human placental tissue with Apoptosis inhibitor 5 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
IHC staining of FFPE rat intestine tissue with Apoptosis inhibitor 5 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
IHC staining of FFPE mouse intestine tissue with Apoptosis inhibitor 5 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with Apoptosis inhibitor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of frozen rat heart tissue with Apoptosis inhibitor 5 antibody.
ICC staining of human HeLa cells with Apoptosis inhibitor 5 antibody.
Immunofluorescent staining of FFPE human SK-O-V3 cells with Apoptosis inhibitor 5 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of Apoptosis Inhibitor 5 antibody and Lane 1: rat heart; 2: rat brain; 3: rat testis; 4: rat placenta; 5: human MCF-7; 6: human HeLa; 7: human CEM; 8: human SMMC-7721; 9: human COLO320 cell lysate. Predicted molecular weight ~59 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt Q9BZZ5
Localization Nuclear, cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml
Immunohistochemistry (Frozen) : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Limitations This Apoptosis inhibitor 5 antibody is available for research use only.
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Description

Many growth factors and cytokines act as cellular survival factors by preventing programmed cell death(apoptosis). Apoptosis inhibitor 5 (API5) is an antiapoptotic factor which may have a role in protein assembly. The API5 gene to chromosome 11p12 based on an alignment of the API5 sequence with the genomic sequence It is a critical determinant of E2F1-induced apoptosis, acting downstream of E2F to suppress E2F-dependent apoptosis without generally blocking E2F-dependent transcription.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the Apoptosis inhibitor 5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the C-terminus of human Apoptosis inhibitor 5 (amino acids 487-504 of isoform b, KYSSNLGNFNYERSLQGK) was used as the immunogen for this Apoptosis inhibitor 5 antibody.

Storage

After reconstitution, the Apoptosis inhibitor 5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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