Apolipoprotein H Antibody 24A09 / B2GPI Antibody [clone 24A09] (RQ8917)

Apolipoprotein H Antibody 24A09 in Human Liver IHC. Immunohistochemistry staining of FFPE human liver tissue with Apolipoprotein H Antibody clone 24A09 demonstrates widespread granular cytoplasmic staining in hepatocytes. HIER was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing. The hepatocellular distribution is consistent with the liver being a major site of B2GPI production.

Apolipoprotein H Antibody 24A09 in Human Liver Cancer IHC. Immunohistochemistry staining of FFPE human liver cancer tissue with Apolipoprotein H Antibody clone 24A09 demonstrates heterogeneous cytoplasmic staining in malignant hepatocellular cells, with areas of moderate to strong immunoreactivity. HIER was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing. The staining demonstrates detection of B2GPI in the tested liver tumor tissue.

Apolipoprotein H Antibody 24A09 in Mouse Liver IHC. Immunohistochemistry staining of FFPE mouse liver tissue with Apolipoprotein H Antibody clone 24A09 demonstrates broad cytoplasmic staining of hepatocytes. HIER was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing. The staining confirms recognition of mouse B2GPI and complements the documented human reactivity of clone 24A09.

Apolipoprotein H Antibody 24A09 in Human and Mouse Cell Lysates WB. Western blot testing of human HepG2 (lane 1), human Caco-2 (lane 2), and mouse HEPA1-6 (lane 3) cell lysates with Apolipoprotein H Antibody clone 24A09 detects a prominent band at approximately 48-50 kDa, with strongest signal in the HEPA1-6 sample. APOH has a predicted molecular weight of approximately 38 kDa but can migrate at a higher apparent molecular weight due to glycosylation. The observed bands are therefore consistent with detection of glycosylated B2GPI in human and mouse samples.