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Home >> Antibodies >> Apolipoprotein E Antibody / ApoE

Apolipoprotein E Antibody / ApoE (R32471)

  Catalog No Formulation Size Price (USD)  
Image R32471 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 329
Western blot testing of 1) mouse spleen and 2) mouse kidney lysate with APOE antibody at 0.5ug/ml. Predicted molecular weight: 34-37 kDa.
IHC testing of FFPE mouse liver with APOE antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
IHC testing of FFPE rat liver with APOE antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P08226
Localization Cytoplasmic, membranous
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 1-2ug/ml
Limitations This Apolipoprotein E antibody is available for research use only.
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Description

ApoE is also known as AD2 or LPG. The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Alternative splicing results in multiple transcript variants.

Application Notes

Optimal dilution of the Apolipoprotein E antibody should be determined by the researcher.

Immunogen

Amino acids D55-Q294 were used as the immunogen for the Apolipoprotein E antibody.

Storage

Prior to reconstitution, store at 4oC. After reconstitution, the Apolipoprotein E antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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