APOH Antibody APOH/3705 / Beta-2-Glycoprotein I Antibody [clone APOH/3705] (V5408)

APOH Antibody APOH/3705 Protein Microarray Validation. Protein microarray analysis of APOH Antibody clone APOH/3705 against more than 19,000 full-length human proteins identifies apolipoprotein H as the top-ranked target with a Z-score of 154.59 and an S-score of 150.59. The next-ranked protein produces a Z-score of only 4.29, demonstrating exceptional separation of the intended target from other array proteins. The Z-score measures signal strength in standard deviations above the array mean, while the S-score measures separation from the next-ranked protein. This Beta-2-Glycoprotein I Antibody demonstrates highly selective recognition of APOH in the HuProt protein microarray.

APOH Antibody APOH/3705 in Human Liver IHC. Immunohistochemistry staining of FFPE human liver tissue with APOH Antibody clone APOH/3705 demonstrates prominent granular cytoplasmic staining in hepatocytes, with variable staining intensity among individual cells. HIER was performed by boiling tissue sections in pH 9, 10 mM Tris with 1 mM EDTA for 20 min followed by cooling before testing. The observed hepatocellular staining is consistent with the liver being a major site of beta-2-glycoprotein I production.

APOH Antibody APOH/3705 Purity and Integrity SDS-PAGE. SDS-PAGE analysis of 2 ug purified, BSA-free APOH Antibody clone APOH/3705 under non-reducing (NR) and reducing (R) conditions. The non-reduced antibody migrates predominantly at approximately 140-150 kDa, while reduction produces a prominent heavy-chain band near 50 kDa and a lighter chain band near 25 kDa. The expected immunoglobulin banding pattern supports the purity and structural integrity of this Beta-2-Glycoprotein I Antibody preparation.

APOH Antibody APOH/3705 in Human Liver WB. Western blot analysis of human liver tissue lysate with APOH Antibody clone APOH/3705 detects a strong, discrete band at approximately 45-50 kDa, with minimal additional reactivity under the tested conditions. The observed migration is consistent with glycosylated beta-2-glycoprotein I, a plasma glycoprotein with a mature apparent molecular weight higher than that predicted from its polypeptide sequence alone.