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Home >> Antibodies >> APOC3 Antibody / Apolipoprotein C III

APOC3 Antibody / Apolipoprotein C III (R32511)

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  Catalog No Formulation Size Price (USD)  
Image R32511 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) rat liver and 2) mouse liver lysate with APOC3 antibody at 0.5ug/ml. Predicted molecular weight 9-11 kDa.
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Western blot testing of 1) rat liver and 2) mouse liver lysate with APOC3 antibody at 0.5ug/ml. Predicted molecular weight 9-11 kDa.
Flow cytometry testing of human U-2 OS cells with APOC3 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= APOC3 antibody.
Flow cytometry testing of human SiHa cells with APOC3 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= APOC3 antibody.
Western blot testing of 1) rat liver, 2) rat RH35, 3) mouse liver and 4) mouse HEPA1-6 cell lysate with APOC3 antibody at 0.5ug/ml. Predicted molecular weight 9-11 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P02656
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This APOC3 antibody is available for research use only.
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Description

Apolipoprotein C-III, also known as apo-CIII, is a protein that in humans is encoded by the APOC3 gene. Apo-CIII is a component of very low density lipoprotein (VLDL). APOC3 inhibits lipoprotein lipase and hepatic lipase; it is thought to delay catabolism of triglyceride-rich particles. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the A-1 and C-III genes are convergently transcribed. An increase in apoC-III levels induces the development of hypertriglyceridemia.

Application Notes

Differences in protocols and secondary/substrate sensitivity may require the APOC3 antibody to be titrated for optimal performance.

Immunogen

Amino acids S21-A99 from the human protein were used as the immunogen for the APOC3 antibody.

Storage

After reconstitution, the APOC3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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