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Home >> Antibodies >> APOBEC3G Antibody

APOBEC3G Antibody (R32470)

  Catalog No Formulation Size Price (USD)  
Image R32470 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 329
Western blot testing of 1) human A431 and 2) human Jurkat cell lysate with APOBEC3G antibody at 0.5ug/ml. Predicted molecular weight: ~46 kDa.
IHC testing of FFPE human pancreatic cancer tissue with APOBEC3G antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
IHC testing of FFPE human tonsil with APOBEC3G antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
Flow cytometry testing of human THP-1 cells with APOBEC3G antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=APOBEC3G antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt Q9HC16
Localization Cytoplasmic, membranous
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 1-2ug/ml
Flow Cytometry : 1-3ug/10^6 cells
Limitations This APOBEC3G antibody is available for research use only.
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Description

APOBEC3G (Apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G) is a human enzyme encoded by the APOBEC3G gene. This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. The protein encoded by this gene has been found to be a specific inhibitor of human immunodeficiency virus-1 (HIV-1) infectivity.

Application Notes

Optimal dilution of the APOBEC3G antibody should be determined by the researcher.

Immunogen

Amino acids E191-N384 were used as the immunogen for the APOBEC3G antibody.

Storage

Prior to reconstitution, store at 4oC. After reconstitution, the APOBEC3G antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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