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Home >> Antibodies >> APOA1 Antibody

APOA1 Antibody [clone APOA1/3661] (V8388)

  Catalog No Formulation Size Price (USD)  
Image V8388-100UG 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 429
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V8388-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V8388SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
IHC staining of FFPE human liver with APOA1 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver with APOA1 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free APOA1 antibody as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name APOA1/3661
Purity Protein G affinity chromatography
UniProt P02647
Localization Secreted
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This APOA1 antibody is available for research use only.
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Description

Apolipoproteins are protein components of plasma lipoproteins. The human apoA-I gene encodes a single chain, 243 amino acid protein which promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A-I is the major protein component of high density lipoprotein (HDL) in the plasma. It can function as a cofactor for lecithin cholesterolacyltransferase (LCAT), which is responsible for the formation of most plasma cholesteryl esters. The human apoA-II gene encodes the second most abundant protein of HDL particles, where it influences plasma levels of free fatty acids (FFA). The human apoA-IV gene encodes a 396 amino acid preprotein, which after proteolytic processing is secreted from the intestine in association with chylomicron particles. ApoA-IV is a potent activator of LCAT in vitro. The human apoA-V gene encodes a 366 amino acid protein that is believed to be an important determinant of plasma triglyceride levels.

Application Notes

Optimal dilution of the APOA1 antibody should be determined by the researcher.

Immunogen

A portion of amino acids 83-167 from the human protein was used as the immunogen for the APOA1 antibody.

Storage

Store the APOA1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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