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Home >> Antibodies >> APLP1 Antibody (C-Terminal Region)

APLP1 Antibody (C-Terminal Region) (F54145)

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  Catalog No Formulation Size Price (USD)  
Image F54145-0.2ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.2 ml 399
Image
F54145-0.05ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.05 ml 185
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Western blot testing of human 1) U-251 MG and 2) U-87 MG cell lysate with APLP1 antibody at 1:2000. Expected molecular weight: 76 kDa (unmodified), ~86 kDa (soluble/glycosylated form), 92~95 kDa (mature form).
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Western blot testing of human 1) U-251 MG and 2) U-87 MG cell lysate with APLP1 antibody at 1:2000. Expected molecular weight: 76 kDa (unmodified), ~86 kDa (soluble/glycosylated form), 92~95 kDa (mature form).
Flow cytometry testing of fixed and permeabilized human U-87 MG cells with APLP1 antibody; Blue=isotype control, Green= APLP1 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P51693
Applications Western blot : 1:1000-1:2000
Flow cytometry : 1:25 per million cells
Limitations This APLP1 antibody is available for research use only.
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Description

Amyloid-like protein 1 may play a role in postsynaptic function. The C-terminal gamma-secretase processed fragment, ALID1, activates transcription activation through APBB1 (Fe65) binding (By similarity). Couples to JIP signal transduction through C-terminal binding. May interact with cellular G-protein signaling pathways. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I.

Application Notes

The stated application concentrations are suggested starting points. Titration of the APLP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 505-539 from human Amyloid-like protein 1 was used as the immunogen for the APLP1 antibody.

Storage

Aliquot the APLP1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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