- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
APH1A Antibody / Gamma-secretase subunit APH-1A (FY12980)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
FY12980 | Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml | 100 ug | 439 |
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APH1A antibody detects Gamma-secretase subunit APH-1A, a multi-pass membrane protein essential for the assembly and activity of the gamma-secretase complex. The UniProt recommended name is Gamma-secretase subunit APH-1A (APH1A), also known as Anterior pharynx-defective 1A homolog. Gamma-secretase is an intramembrane protease complex responsible for cleaving type I transmembrane proteins such as amyloid precursor protein (APP) and Notch, generating signaling fragments involved in development and neurodegeneration.
Functionally, APH1A antibody identifies a 265-amino-acid protein that serves as a structural scaffold for the gamma-secretase complex. APH1A forms stable interactions with Presenilin (PSEN1 or PSEN2), Nicastrin (NCSTN), and PEN2, facilitating proper folding, assembly, and trafficking of the complex. This assembly is required for the intramembrane proteolytic processing of substrates like APP, which produces amyloid-beta peptides linked to Alzheimer's disease pathogenesis. APH1A is also essential for Notch receptor cleavage, influencing cell fate determination and tissue differentiation.
The APH1A gene is located on chromosome 1q21.2 and encodes a protein localized to the endoplasmic reticulum and Golgi apparatus before its incorporation into the mature gamma-secretase complex at the plasma membrane. It contains seven transmembrane helices and conserved motifs necessary for Presenilin binding and enzymatic activity. APH1A is one of two APH1 homologs in humans, the other being APH1B, which participates in distinct gamma-secretase isoforms with varying substrate preferences and tissue expression patterns.
Mutations or dysregulation of APH1A disrupt gamma-secretase assembly and reduce proteolytic activity, leading to defects in Notch signaling and accumulation of unprocessed APP. These abnormalities contribute to neurodevelopmental disorders and neurodegenerative diseases, including Alzheimer's disease. Experimental studies show that selective regulation of APH1A-containing gamma-secretase complexes can modulate amyloid-beta production without affecting Notch cleavage, providing a potential therapeutic strategy.
APH1A antibody is widely used in neurobiology, signal transduction, and protein trafficking research. It is suitable for immunoblotting, immunohistochemistry, and co-immunoprecipitation to examine gamma-secretase composition and function. In Alzheimer's research, APH1A detection helps analyze isoform-specific gamma-secretase activity and its modulation by small molecules or genetic factors.
Structurally, APH1A acts as a transmembrane scaffold stabilizing the Presenilin catalytic core and maintaining the architecture of the protease complex. Its interactions are regulated by lipid environment and post-translational modifications such as glycosylation. NSJ Bioreagents provides APH1A antibody reagents validated for use in gamma-secretase assembly, Alzheimer's disease, and Notch signaling research.
Optimal dilution of the APH1A antibody should be determined by the researcher.
A synthetic peptide corresponding to a sequence at the C-terminus of human APH1A was used as the immunogen for the APH1A antibody.
After reconstitution, the APH1A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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