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Home >> Antibodies >> APG4C Antibody / ATG4C

APG4C Antibody / ATG4C (RQ7548)

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  Catalog No Formulation Size Price (USD)  
Image RQ7548 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human U-2 OS cells with APG4C antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human U-2 OS cells with APG4C antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 293T cell lysate with APG4C antibody. Predicted molecular weight ~52 kDa.
Flow cytometry testing of human U-251 cells with APG4C antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= APG4C antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q96DT6
Localization Nuclear, cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This APG4C antibody is available for research use only.
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Description

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized.

Application Notes

Optimal dilution of the APG4C antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids M1-L457) was used as the immunogen for the APG4C antibody.

Storage

After reconstitution, the APG4C antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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